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KMID : 0545120060160111728
Journal of Microbiology and Biotechnology
2006 Volume.16 No. 11 p.1728 ~ p.1733
Sphingobacterium composti sp. nov. a Novel DNase-Producing Bacterium Isolated from Compost
Ten Leonid N.

Liu Qing-Mei
Im Wan-Taek
Aslam Zubair
Lee Sung-Taik
Abstract
A Gram-negative, strictly aerobic, nonmotile, and nonspore-forming bacterial strain, designated T5-12T, was isolated from compost and characterized using a polyphasic taxonomical approach. The isolate was positive for catalase and oxidase tests. It could degrade DNA, but was negative for degradation of macromolecules such as casein, collagen, starch, chitin, cellulose, and xylan. The DNA G+C content was 36.0 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major fatty acids were iso-C15:0 (45.6%), iso-C17:0 3OH (17.2%), and summed feature 4 (C16:1¥ø7c and/or iso-C15:0 2OH, 14.9%). Comparative 16S rRNA gene sequence analysis showed that strain T5-12T fell within the radiation of the cluster comprising members of the genus Sphingobacterium. Strain T5-12T exhibited lower than 94% of 16S rRNA gene sequence similarity with respect to the type strains of recognized Sphingobacterium species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain T5-12T (=KCTC 12578T=LMG 23401T=CCUG 52467T) should be classified in the genus Sphingobacterium as the type strain of a novel species, for which the name Sphingobacterium
composti sp. nov. is proposed.
KEYWORD
Sphingobacterium composti sp. nov, compost, polyphasic taxonomy
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